Over the last decades, organisations have increasingly been more knowledge intensive and professional. Professional work generally has certain specific properties related to its working context. In this paper, a case study conducted within emergency healthcare, in which professional groups like for example physicians and nurses were the objects of study, will be presented. This paper explores different characteristics of these professions in the time and life critical work practice performed within emergency healthcare. The aim of this paper is partly to analyse specific characteristics of the healthcare professions and their use of information systems, partly to identify the implications and challenges that the professions face while using information systems. Characteristics related to a certain healthcare profession can be seen as having an impact on its professionals' attitudes and use of information systems, both on an individual and on a collective level. Some challenges in the use of information systems can also be due to how the development of the system and implementation processes are organised.