One of the most common titanium alloys for aerospace industry is Ti-6Al-4V (usually designed as Ti-64) which is used for manufacturing aero-engine components, such as fan discs, compressor discs, blades andstators. The maximum service temperature for this alloy is limited partly because of degradation of mechanical properties at elevated temperatures (above 480 ºC). During the first stage of oxidation the oxidescale is protective, whereas after prolonged oxidation time it loses its protective nature and favours higher diffusion of oxygen through the oxide. In the present study, cyclic thermal treatments were performed in air at 500 and 700 ºC, up to 500 hours, and compared with similar studies carried out on isothermal oxidation conditions. The evolution of the surface oxidation was analyzed by metallographic techniques and X-ray diffraction, together with a detailed advanced characterization of the microstructure by Scanning Electron Microscopy and Focus Ions Beam. The results point out that the cyclic thermal treatments induced a strong increase of the weight gain compared to isothermal treatments. The analysis of the oxide scale revealed not only the presence of rutile, at 700 ºC, but also anatase and TiOx at 500 ºC for both isothermal and cyclic thermal treatments. At 700 ºC, thermal stress caused by cyclic thermal treatments promoted the fracture of the oxide after the first 20 hours.