Manufacturing and automation lines are getting expanded to include Digital Twins as stepstowards Industry 4.0. In this regard, apart from the issues that Digital Twin designers commonly experience during the process, implementing the virtual model on the real system while transferring the codes and data can be challenging as well. Using Digital Twin as part of the future laboratory facility of University West for the automation line at Production Technology Centre (PTC), it first needs to be tested on the real line to discover possible problems that future students might face. In this master thesis, these errors and faults are investigated to help the automation line staff and students avoid them by possible solutions.The assembly routine in the Digital Twin of the automation line was tested with an HMI using the OPCUA platform both in manual mode and automatic mode. The automation line was tested in manual mode by changing the function blocks which work with the real PLC. Moreover, the students can use the conversion table to understand the difference between the Digital Twin and its real entity function blocks. However, it can take much time for students to make their HMI and PLC code compatible with the automation line at PTC. As a result, suggestions are made, and attention to some minor details of the test procedure are emphasized. Although, due to the COVID-19 pandemic, and packed schedule of PTC staff, the automation line was not completely assembled and for safety reasons the automatic mode of the tests was withdrawn.